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j558l cells  (ATCC)


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    ATCC j558l cells
    RBD trimer is the most potent in triggering BCR signaling (A) Synaptic accumulation of BCRs triggered by native RBD, RBD trimer, and native spike on the surface of the <t>J558L</t> B cell line expressing SARS-CoV-2 antibody 2F6-IgG-BCRs captured by a confocal microscopy. The magnified insets (lower right corner) show the highlighted cells in the original image within the white boxes. The BCR molecules are labelled in red, and the scale bar in the inset window represents 1.5 μm. (B) Synaptic recruitment of phosphorylated Syk (pSyk) to the contact area of a single J558L B cell after BCR accumulation by native RBD, RBD trimer, and native spike captured by a confocal microscopy. The BCRs and pSyk molecules are respectively labelled in red and green, and their merged images are also shown. The scale bar represents 1.5 μm. (C–F) Statistical analysis for the contact area and mean fluorescence intensity (MFI) of BCRs (C and D) and pSky (E and F) recruited in the immunological synapses on the surface of J558L-2F6-IgG-BCRs B cells. Each dot in the plot represents the data of one cell with indicated means and standard deviation. (G) Fluorescence intensity profiles from (B) showing degrees of co-localization between BCR and pSyk on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. (H) Pearson correlation index showing the spatial distribution between BCR and pSky microclusters on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. Each dot represents an individual measurement from a single B cell. Bars indicate the mean values and standard deviations from at least three independent experiments. Statistical significance was analyzed using unpaired Students’ t -test (two-tailed) (∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001).
    J558l Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/j558l cells/product/ATCC
    Average 93 stars, based on 93 article reviews
    j558l cells - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "RBD trimer mRNA vaccine elicits broad and protective immune responses against SARS-CoV-2 variants"

    Article Title: RBD trimer mRNA vaccine elicits broad and protective immune responses against SARS-CoV-2 variants

    Journal: iScience

    doi: 10.1016/j.isci.2022.104043

    RBD trimer is the most potent in triggering BCR signaling (A) Synaptic accumulation of BCRs triggered by native RBD, RBD trimer, and native spike on the surface of the J558L B cell line expressing SARS-CoV-2 antibody 2F6-IgG-BCRs captured by a confocal microscopy. The magnified insets (lower right corner) show the highlighted cells in the original image within the white boxes. The BCR molecules are labelled in red, and the scale bar in the inset window represents 1.5 μm. (B) Synaptic recruitment of phosphorylated Syk (pSyk) to the contact area of a single J558L B cell after BCR accumulation by native RBD, RBD trimer, and native spike captured by a confocal microscopy. The BCRs and pSyk molecules are respectively labelled in red and green, and their merged images are also shown. The scale bar represents 1.5 μm. (C–F) Statistical analysis for the contact area and mean fluorescence intensity (MFI) of BCRs (C and D) and pSky (E and F) recruited in the immunological synapses on the surface of J558L-2F6-IgG-BCRs B cells. Each dot in the plot represents the data of one cell with indicated means and standard deviation. (G) Fluorescence intensity profiles from (B) showing degrees of co-localization between BCR and pSyk on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. (H) Pearson correlation index showing the spatial distribution between BCR and pSky microclusters on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. Each dot represents an individual measurement from a single B cell. Bars indicate the mean values and standard deviations from at least three independent experiments. Statistical significance was analyzed using unpaired Students’ t -test (two-tailed) (∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001).
    Figure Legend Snippet: RBD trimer is the most potent in triggering BCR signaling (A) Synaptic accumulation of BCRs triggered by native RBD, RBD trimer, and native spike on the surface of the J558L B cell line expressing SARS-CoV-2 antibody 2F6-IgG-BCRs captured by a confocal microscopy. The magnified insets (lower right corner) show the highlighted cells in the original image within the white boxes. The BCR molecules are labelled in red, and the scale bar in the inset window represents 1.5 μm. (B) Synaptic recruitment of phosphorylated Syk (pSyk) to the contact area of a single J558L B cell after BCR accumulation by native RBD, RBD trimer, and native spike captured by a confocal microscopy. The BCRs and pSyk molecules are respectively labelled in red and green, and their merged images are also shown. The scale bar represents 1.5 μm. (C–F) Statistical analysis for the contact area and mean fluorescence intensity (MFI) of BCRs (C and D) and pSky (E and F) recruited in the immunological synapses on the surface of J558L-2F6-IgG-BCRs B cells. Each dot in the plot represents the data of one cell with indicated means and standard deviation. (G) Fluorescence intensity profiles from (B) showing degrees of co-localization between BCR and pSyk on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. (H) Pearson correlation index showing the spatial distribution between BCR and pSky microclusters on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. Each dot represents an individual measurement from a single B cell. Bars indicate the mean values and standard deviations from at least three independent experiments. Statistical significance was analyzed using unpaired Students’ t -test (two-tailed) (∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001).

    Techniques Used: Expressing, Confocal Microscopy, Fluorescence, Standard Deviation, Two Tailed Test


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Mutagenesis, Luciferase, Enzyme-linked Immunospot, Digital PCR, Expressing, Plasmid Preparation, Software



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    RBD trimer is the most potent in triggering BCR signaling (A) Synaptic accumulation of BCRs triggered by native RBD, RBD trimer, and native spike on the surface of the <t>J558L</t> B cell line expressing SARS-CoV-2 antibody 2F6-IgG-BCRs captured by a confocal microscopy. The magnified insets (lower right corner) show the highlighted cells in the original image within the white boxes. The BCR molecules are labelled in red, and the scale bar in the inset window represents 1.5 μm. (B) Synaptic recruitment of phosphorylated Syk (pSyk) to the contact area of a single J558L B cell after BCR accumulation by native RBD, RBD trimer, and native spike captured by a confocal microscopy. The BCRs and pSyk molecules are respectively labelled in red and green, and their merged images are also shown. The scale bar represents 1.5 μm. (C–F) Statistical analysis for the contact area and mean fluorescence intensity (MFI) of BCRs (C and D) and pSky (E and F) recruited in the immunological synapses on the surface of J558L-2F6-IgG-BCRs B cells. Each dot in the plot represents the data of one cell with indicated means and standard deviation. (G) Fluorescence intensity profiles from (B) showing degrees of co-localization between BCR and pSyk on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. (H) Pearson correlation index showing the spatial distribution between BCR and pSky microclusters on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. Each dot represents an individual measurement from a single B cell. Bars indicate the mean values and standard deviations from at least three independent experiments. Statistical significance was analyzed using unpaired Students’ t -test (two-tailed) (∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001).
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    RBD trimer is the most potent in triggering BCR signaling (A) Synaptic accumulation of BCRs triggered by native RBD, RBD trimer, and native spike on the surface of the <t>J558L</t> B cell line expressing SARS-CoV-2 antibody 2F6-IgG-BCRs captured by a confocal microscopy. The magnified insets (lower right corner) show the highlighted cells in the original image within the white boxes. The BCR molecules are labelled in red, and the scale bar in the inset window represents 1.5 μm. (B) Synaptic recruitment of phosphorylated Syk (pSyk) to the contact area of a single J558L B cell after BCR accumulation by native RBD, RBD trimer, and native spike captured by a confocal microscopy. The BCRs and pSyk molecules are respectively labelled in red and green, and their merged images are also shown. The scale bar represents 1.5 μm. (C–F) Statistical analysis for the contact area and mean fluorescence intensity (MFI) of BCRs (C and D) and pSky (E and F) recruited in the immunological synapses on the surface of J558L-2F6-IgG-BCRs B cells. Each dot in the plot represents the data of one cell with indicated means and standard deviation. (G) Fluorescence intensity profiles from (B) showing degrees of co-localization between BCR and pSyk on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. (H) Pearson correlation index showing the spatial distribution between BCR and pSky microclusters on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. Each dot represents an individual measurement from a single B cell. Bars indicate the mean values and standard deviations from at least three independent experiments. Statistical significance was analyzed using unpaired Students’ t -test (two-tailed) (∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001).
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    Image Search Results


    RBD trimer is the most potent in triggering BCR signaling (A) Synaptic accumulation of BCRs triggered by native RBD, RBD trimer, and native spike on the surface of the J558L B cell line expressing SARS-CoV-2 antibody 2F6-IgG-BCRs captured by a confocal microscopy. The magnified insets (lower right corner) show the highlighted cells in the original image within the white boxes. The BCR molecules are labelled in red, and the scale bar in the inset window represents 1.5 μm. (B) Synaptic recruitment of phosphorylated Syk (pSyk) to the contact area of a single J558L B cell after BCR accumulation by native RBD, RBD trimer, and native spike captured by a confocal microscopy. The BCRs and pSyk molecules are respectively labelled in red and green, and their merged images are also shown. The scale bar represents 1.5 μm. (C–F) Statistical analysis for the contact area and mean fluorescence intensity (MFI) of BCRs (C and D) and pSky (E and F) recruited in the immunological synapses on the surface of J558L-2F6-IgG-BCRs B cells. Each dot in the plot represents the data of one cell with indicated means and standard deviation. (G) Fluorescence intensity profiles from (B) showing degrees of co-localization between BCR and pSyk on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. (H) Pearson correlation index showing the spatial distribution between BCR and pSky microclusters on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. Each dot represents an individual measurement from a single B cell. Bars indicate the mean values and standard deviations from at least three independent experiments. Statistical significance was analyzed using unpaired Students’ t -test (two-tailed) (∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001).

    Journal: iScience

    Article Title: RBD trimer mRNA vaccine elicits broad and protective immune responses against SARS-CoV-2 variants

    doi: 10.1016/j.isci.2022.104043

    Figure Lengend Snippet: RBD trimer is the most potent in triggering BCR signaling (A) Synaptic accumulation of BCRs triggered by native RBD, RBD trimer, and native spike on the surface of the J558L B cell line expressing SARS-CoV-2 antibody 2F6-IgG-BCRs captured by a confocal microscopy. The magnified insets (lower right corner) show the highlighted cells in the original image within the white boxes. The BCR molecules are labelled in red, and the scale bar in the inset window represents 1.5 μm. (B) Synaptic recruitment of phosphorylated Syk (pSyk) to the contact area of a single J558L B cell after BCR accumulation by native RBD, RBD trimer, and native spike captured by a confocal microscopy. The BCRs and pSyk molecules are respectively labelled in red and green, and their merged images are also shown. The scale bar represents 1.5 μm. (C–F) Statistical analysis for the contact area and mean fluorescence intensity (MFI) of BCRs (C and D) and pSky (E and F) recruited in the immunological synapses on the surface of J558L-2F6-IgG-BCRs B cells. Each dot in the plot represents the data of one cell with indicated means and standard deviation. (G) Fluorescence intensity profiles from (B) showing degrees of co-localization between BCR and pSyk on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. (H) Pearson correlation index showing the spatial distribution between BCR and pSky microclusters on J558L-2F6-IgG-BCRs B cells after engagement with native RBD, RBD trimer, and native spike. Each dot represents an individual measurement from a single B cell. Bars indicate the mean values and standard deviations from at least three independent experiments. Statistical significance was analyzed using unpaired Students’ t -test (two-tailed) (∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001).

    Article Snippet: J558L cells , ATCC , TIB-6.

    Techniques: Expressing, Confocal Microscopy, Fluorescence, Standard Deviation, Two Tailed Test

    Journal: iScience

    Article Title: RBD trimer mRNA vaccine elicits broad and protective immune responses against SARS-CoV-2 variants

    doi: 10.1016/j.isci.2022.104043

    Figure Lengend Snippet:

    Article Snippet: J558L cells , ATCC , TIB-6.

    Techniques: Virus, Recombinant, Mutagenesis, Luciferase, Enzyme-linked Immunospot, Digital PCR, Expressing, Plasmid Preparation, Software